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KMID : 0364820200560030277
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Korean Journal of Microbiology
2020 Volume.56 No. 3 p.277 ~ p.284
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Electric and electromagnetic effects on growth and biofilm formation of Pseudomonas aeruginosa
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Kim Se-Jin
Shin Young-Sun
Park Shin-Hae
Im Su-Jin
Hwang Hyeon-Ji
Kim Soo-Kyoung
Lee Joon-Hee
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Abstract
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Bacterial growth and biofilm formation mediate serious infections in human and cause many problems in civil and industrial facilities. In this study, electricity and magnetic field have been tested to modulate bacterial growth and biofilm formation. As model bacteria, a common model bacterium Escherichia coli, a human pathogen Pseudomonas aeruginosa that is a notorious biofilm-former, and a pus-forming pathogen Staphylococcus aureus were used. When direct current electricity was applied, there was no effect at 1.5 V, but bacterial growth was suppressed at 3 V, and growth was more strongly suppressed on the cathode side. To know the magnetic field effect on biofilm formation, while P. aeruginosa was forming biofilm in flow-cell system, magnetic field was given by magnet or solenoid wound around the flow-cell chamber. The magnitude of magnetic field was adjusted by changing the number of coils of the solenoid and other factors such as current and voltage were fixed. Our results obtained from the observation and quantification by confocal microscopy showed that the magnetic field’s effect on biofilm formation of P. aeruginosa varies depending on the magnitude of the field. Although strong magnetic fields might inhibit biofilm formation, our results showed that biofilm formation increased when a weak magnetic field is given.
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KEYWORD
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Pseudomonas aeruginosa, biofilm, formation, electricity, flow-cell, magnetic field
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